Short-term nitrate (nitrite) inhibition of nitrogen fixation in Azotobacter chroococcum

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Short-term nitrate (nitrite) inhibition of nitrogen fixation in Azotobacter chroococcum.

Nitrate-grown Azotobacter chroococcum ATCC 4412 cells lack the ability to fix N2. Nitrogenase activity developed after the cells were suspended in a combined nitrogen-free medium and was paralleled by a concomitant decrease in nitrate assimilation capacity. In such treated cells exhibiting transitory nitrate assimilation and N2-fixation capacity, nitrate or nitrite caused a short-term inhibitor...

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Symbiosis between Chlorella Sp. and Azotobacter Chroococcum and Nitrogen Fixation

Alfred Koch has given considerable attention to the subject of nitrogen fixation by bacteria as resulting from their symbiosis with alga~. Many others have also investigated various features of this problem. In spite of all this there are numerous questions which remain unsolved. The use of soil cultures for such studies is objectionable on account of the soil's complexity, its variety of flora...

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Relationship of nitrite and hydroxylamine reductases to nitrate assimilation and nitrogen fixation in Azotobacter agile.

The nitrogen fixation process has been recently reviewed by Wilson (1952), Burris (1956), Shug et al. (1956), and Gest et al. (1956). Although the characteristics of the over-all process in vivo are well known, neither the pathway nor the enzymatic steps has been established. On the other hand, a number of enzymes from bacteria, fungi and higher plants have been characterized which together can...

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Reduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum.

1. The assimilatory nitrite reductase of the N(2)-fixing bacterium Azotobacter chroococcum was prepared in a soluble form from cells grown aerobically with nitrate as the nitrogen source, and some of its properties have been studied. 2. The enzyme is a FAD-dependent metalloprotein (mol.wt. about 67000), which stoicheiometrically catalyses the direct reduction of nitrite to NH(3) with NADH as th...

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Nitrogen Fixation by Azotobacter vinelandii

Nitrogenase was isolated and purified from wildtype and a tungsten-resistant mutant (LM2) of Azotobacter vinelandii strain OP derepressed on medium containing 1-10 mM W. While the enzyme from the wild-type strain contained the polypeptides of the conventional enzyme, metal analysis of component 1 demonstrated the existence of one atom each of molybdenum and tungsten. Furthermore, the ESR spectr...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1986

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.165.1.240-243.1986